Receptor binding techniques /
edited by Anthony P. Davenport.
- 2nd ed.
- Totowa, N.J. : Humana Press, c2005.
- x, 240 p. : ill. ; 33 cm.
- Methods in molecular biology ; v. 306 .
- Methods in molecular biology (Clifton, N.J.) ; v. 306. .
Includes bibliographical references and index.
Receptor databases and computational websites for ligand binding -- Identification of orphan g protein-coupled receptor ligands using fluorometric imaging plate reader assays -- Quantitative analysis of orphan g protein-coupled receptor -- Mrnas by taqman real-time pcr: g2a and gpr4 lysophospholipid receptor expression in -- Leucocytes and in a rat myocardial infarction-heart model -- -- Mrna detection by in situ and northern hybridization -- Radioligand binding and molecular imaging techniques for the quantitative analysis of established and emerging orphan receptor systems -- Measurement of radioligand binding by scintillation proximity assay -- Autoradiography of enzymes, second messenger systems, and ion channels -- Immunocytochemical localization of receptors using light and confocal microscopy with applications to the phenotypic characterization of knockout mice -- live cell imaging of g protein-coupled receptors -- imaging and characterization of radioligands for positron emission tomography using quantitative phosphor imaging autoradiography -- dynamic in vivo imaging of receptors in small animals using positron emission tomography.